Sequence Specific Retention Calculator Version 3.X ©2010 MB Centre for Proteomics & Systems Biology

1. Define the peptide sequences for the model. Sequences

2. Enter the correlation data to calculate Retention time or Hydrophobicity Index (HI)      Retention time = a + b * hydrophobicity or      HI = a + b * hydrophobicity LEARN ABOUT NEW HYDROPHOBICITY (HI) SCALES (Krokhin OV, Spicer V. Anal Chem. 81(22) 9522-30 (2009)) a:     b:

3. Select Separation System 300Å C18 column, 0.1% TFA HI = -0.231 + 0.6343 * H 100Å C18 column, 0.1% TFA HI = 3.1551 + 0.5835 * H 100Å C18 column at pH 10 HI = 0.0663 + 0.5195 * H 100Å C18 column, 0.1% Formic Acid 100Å C18 column, 0.1% Formic Acid 2010 HI = -2.6687 + 0.4954 * H

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NEW SEQUENCE SPECIFIC SLOPE CALCULATOR (SSSCalc)
Calculates Slopes S for Peptide RP-HPLC's
Linear Solvent Strength (LSS) Theory

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